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. 2014 Mar 5;28(4):565–574. doi: 10.1210/me.2013-1293

Figure 1.

Figure 1.

DNMT3b expression is associated with macrophage polarization in obesity. A, The expression of DNMT3b, but not DNMT3a, is decreased in F4/80+CD11c−CD206+ M2 ATMs more than that in F4/80+CD11c+CD206− M1 ATMs. B and C, The expression of DNMT3b, but not DNMT3a, is elevated in ATMs isolated from ob/ob (B) and diet-induced obese mice (C). Isolation of M1/M2 ATMs and total ATMs was conducted as described in Materials and Methods. LF: low fat diet, HF: high fat diet (n = 4/group). D–H, Stearate stimulates DNMT3b mRNA (D) and protein (E) levels, whereas docosahexaenoic acid (DHA) (F) inhibits DNMT3b mRNA and IL-4 inhibits both DNMT3b mRNA (G) and protein (H) levels in macrophages. Raw264.7 macrophages were treated with BSA, stearate (C:18, 200 μM), DHA (50 μM), or IL-4 (10 ng/mL) for 48 hours (n = 6/group). Gene expression was measured by real-time RT-PCR and normalized to cyclophilin. Protein levels were measured by immunoblotting. All data are expressed as mean ± SEM; *, P < .05.