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. 2014 Mar 5;28(4):565–574. doi: 10.1210/me.2013-1293

Figure 6.

Figure 6.

DNMT3b regulates DNA methylation at PPARγ1 promoter. A and B, DNMT3b knockdown decreases DNA methylation at PPARγ1 promoter. A, Methylation levels at CpG sites corresponding to 15–29 on PPARγ1 promoter (see Figure 5A). B, Average methylation levels of all these CpG sites. DNMT3b siRNA knockdown in Raw264.7 macrophages was conducted as described in Figure 2. CpG methylation was measured by pyrosequencing as described in Materials and Methods. All data are expressed as mean ± SEM (n = 4/group0; *, P < .05 vs control. 3bKD, DNMT3b knockdown. C, DNMT3b knockdown prevents stearate-induced increase of PPARγ1 promoter methylation. All data are expressed as mean ± SEM (n = 4/group); #, P < .05 vs control or 3bKD+C:18. 3bKD, DNMT3b knockdown.