Expression of Notch ligands, receptors, and target genes in the embryonic ovary. Embryonic ovaries from CD1 mice were collected from timed matings at E13.5 to PND0. Multiple ovaries were pooled to create 3 biological replicates that were used for analysis. Levels of each gene are plotted relative to 18S rRNA to show the comparative expression of Notch ligands, receptors, and target genes at each time point. For each gene, letters are used to indicate statistical differences (P < .05) in the level of expression compared with adjacent time points.