Figure 5.

A minimal rGH promoter is required for SSTR3-dependent rGH transcriptional repression. A and B, Relative baseline Pit-1 mRNA expression (A, n = 3), and protein levels (B, representative blot) in untreated GpSSTR3^WT and GpCon. C, Schematic diagram of the full-length (rGH1.7) rGH promoter as well as deletion constructs (rGHP220 and rGHP160); transcription factor binding sites are indicated. D, Relative promoter activity of rGH promoter deletion constructs in untreated GpSSTR3^WT and GpCon (normalized to internal renilla luciferase control) (n = 5).