Figure 1.
Runx1 gene was deleted by Cre recombinase in vivo. DNA isolated from CD11b+ bone marrow cells of 8-week-old Runx1F/F and CD11b-Cre;Runx1F/F mice was used to measure CD11b-Cre–mediated Runx1 gene deletion. After PCR for the Runx1 loci using a 3-primer set (18), PCR products from CD11b+ cells of both Runx1F/F and CD11b-Cre;Runx1F/F mice were run on ethidium bromide–stained 2% agarose gels. F, Runx1 floxed allele; Δ, deleted allele. The percentage of Cre recombination was quantitatively measured using ImageJ software (National Institutes of Health).