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. 2013 Dec 13;63(1):203–215. doi: 10.2337/db13-0384

Figure 1.

Figure 1

Expression of Pak3 and its isoforms in mouse embryonic pancreas. A: RT-qPCR on eYFP+ and eYFP− cells sorted from Ngn3eYFP/+ E15.5 embryonic pancreas revealed that Pak3 is expressed in eYFP+ endocrine cells, whereas Pak1 and Pak2 mRNAs are found in both endocrine (eYFP+) and nonendocrine (eYFP−) populations. B: mRNA structure of the four Pak3 mouse transcripts identified in the brain. Black bars represent primer position for RT-PCR; b and c boxes represent the alternative exons. C: RT-PCR revealing the presence of all known Pak3 isoforms in E15.5 WT embryonic pancreas, with Pak3b being less expressed. A and C: Data are represented as mean ± SD of n = 4. AU, arbitrary units. *P < 0.5; **P < 0.01; ≠, not detected.