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. 2013 Dec 13;63(1):203–215. doi: 10.2337/db13-0384

Figure 2.

Figure 2

Pak3 is expressed in endocrine progenitors and developing endocrine cells in the embryonic pancreas. A–D: In situ hybridization (blue) for Pak3 mRNA revealed expression throughout pancreas development. Dotted line delineates pancreatic domain. In situ hybridization (blue) for Pak3 mRNA followed by immunohistochemistry (brown) or immunofluorescence (red) for different endocrine (E–I) and exocrine (J–L) markers in E15.5 WT embryos, as well as a multipotent progenitor marker at E12.5 (B). Pak3 transcripts are detected exclusively in the endocrine lineage, both in Ngn3+ endocrine progenitors and their descendant cells (Insm1+ or hormone+). Black and white arrows indicate costaining. Brown arrows point to Ptf1a+ multipotent progenitor tip domain. I: Pdx1 low (light brown, *) marks trunk pancreatic progenitors, and Pdx1 high (dark brown, arrows) marks β-cells. Scale bar, 50 (A–D) and 20 μm (E–L). d, duct; a, acini.