Skip to main content
. 2013 Dec 13;63(1):203–215. doi: 10.2337/db13-0384

Figure 3.

Figure 3

Pak3 depends on Ngn3 but is independent of NeuroD. A: RT-qPCR for Pak1–3 and Ngn3 on E15.5 pancreata from Ngn3 KO and control littermates revealed a downregulation of Pak3 but not of Pak1 and -2 in the absence of Ngn3. B: In situ hybridization for Pak3 (blue) followed by ICC (brown) for the pan neuronal marker (Tuj1) or the acinar marker (Cpa1) in E15.5 Ngn3-deficient and control embryos showing ectopic expression of Pak3 in acinar cells (arrows). Scale bars, 50 μm. C: RT-qPCR for Pak1–3 and NeuroD1 on E15.5 pancreata from NeuroD KO and control littermates. D: In situ hybridization for Pak3 and NeuroD1 in E15.5 NeuroD-deficient and control embryos. Pak3 expression is independent of NeuroD. Scale bars, 20 μm. A and C: Data are represented as mean ± SD on n = 4. AU, arbitrary units. **P < 0.01; ***P < 0.001; ≠, not detected.