Pak3 is expressed in enteroendocrine progenitor cells but is not necessary for the differentiation of Glp1- and Gip-expressing L and K cells in the mouse intestine. A: Schematic representation of Affymetrix data comparing expression levels of Ngn3, Pak1, Pak2, and Pak3 in FACS-purified eYFP+ and eYFP− cells from duodenal crypt (n = 3) of Ngn3eYFP/+ adult mice showing specific expression of Pak3 in the enteroendocrine lineage. RT-qPCR for Pak3 (B), Glp1 (C), and Gip (D) on Pak3 WT and Pak3 KO intestinal segments from 3-week-old males (n = 3 for WT and n = 4 for KO). Data are represented as mean ± SD. AU, arbitrary units; Duo, duodenum; Jeju, jejunum; Ill, ileum; Col, colon. *P < 0.05; **P < 0.01; ***P < 0.001.