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RP-HPLC profiles for the crude refolded HsTX1 (A) and HsTX1[R14A] (C), and purified refolded HsTX1 (B) and HsTX1[R14A] (D). The gradient was from 10–40% B in 30 min at a flow rate of 1 mL/min. Buffer A was 0.05% TFA in H2O and Buffer B was 0.05% TFA in MeCN. The A220 was monitored for peak elution.
