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Reversed-phase HPLC analysis after treatment with pepsin (A), α-chymotrypsin (B) and trypsin (C). (D) Haemolysis assay; comparison of the haemolytic ability of HsTX1 and its analogues to haemolysis induced by Triton X-100 indicates that neither HsTX1 nor its analogues induces significant haemolysis. Data shown as average ± SEM (N = 3).
