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Long-term two-photon microscopy does not affect glomerular morphology or nephrin expression. A zebrafish larva (4 dpf) embedded in agarose was fixed after 1 day’s observation time and stained with DAPI (4′,6-diamidino-2-phenylindole) for the nuclei and with an antibody against nephrin. The higher magnification of the glomerulus shows the well-formed glomerular tuft and podocytes expressing nephrin (white box).
