Figure 5.

Stimulation of human podocytes with NaHS causes VEGF release and upregulation of VEGF mRNA. (A) Human podocytes treated with PMA (positive control, 50 ng/ml), PBS (control), 100 µM NaHS, or 1000 µM NaHS. VEGF (pg/ml) release is increased after 120 minutes of stimulation in the 100 and 1000 µM NaHS-treated groups compared with the PBS-treated group. (B) After 24 hours of stimulation with 100 µM NaHS, an increase in VEGF-A mRNA is present compared with PBS (control). Data are presented as the median (IQR). *P<0.05. n=5 per group. Pos, positive control; PMA, phorbol 12-myristate 13-acetate.