Skip to main content
. 2014 Jan 9;25(4):707–716. doi: 10.1681/ASN.2013050452

Figure 5.

Figure 5.

Podocytes are not renewed during aging. (A) Schematic of hNPHS2.rtTA;tetO.Cre;mT/mG mice. (B) Induction protocol. (C) Flow cytometry analysis at 4 weeks and 12 months. Error bars, SEM; n.s., not significant (P=0.55); n>12. mT/mG fluorescent images at (D) 4 weeks and (E) 12 months (arrow, mT-labeled podocyte). Scale bar, 10 µm. (F) Schematic of hPODXL.rtTA;tetO.Cre;mT/mG mice. (G) Flow cytometry analysis at 4 weeks and 12 months. Error bars, SEM; n>6 per group; n.s., not significant (P=1). mT/mG fluorescent images at (H) 4 weeks and (I) 12 months (arrows, mG-labeled podocytes). Scale bar, 10 µm. (J) mG-labeled bone marrow was transplanted into irradiated mT mice. (K) Timeline of procedure. (L) Flow cytometry analysis 4 and 7 months after bone marrow transplant (BMT). Error bar, SEM; n=6 per group; n.s., not significant; P=0.054. mT/mG fluorescent images (M) 4 months and (N) 7 months after BMT (arrows, mG-positive cells). Scale bar, 10 µm. EGFP, enhanced greent fluorescent protein; PCA, core promoter of chicken β-actin.