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. 2014 Jan 9;25(4):693–705. doi: 10.1681/ASN.2013050481

Figure 3.

Figure 3.

Tracing of labeled PECs after induction of glomerular hypertrophy in (A–D) PEC-rtTA/LC1/R26R or (F–H) rtTA/H2B-EGFP reporter mice. (A1–A3) X-gal–stained frozen sections of (A1) control (n=7) and (A2 and A3) experimental remnant kidneys (UNx, n=12; 5/6Nx, n=9). Staining quality is reduced, because no perfusion fixation was performed (for accurate kidney weights). Original magnification, ×400. (A4) β-Gal–positive cells close to the vascular pole (arrows). (B) Statistical analysis of 100 random glomerular cross-sections per animal including data from resected control kidneys (pre-UNx, n=12; pre-5/6Nx, n=13). Virtually no β-gal–positive cells were detected on the glomerular tuft (black bars, glomeruli with β-gal–positive cells within the tuft; error bars, SD). (C) Absolute numbers of β-gal–positive cells exclusively in those glomerular cross-sections showing β-gal positivity on the tuft. Error bars, SD. (D) Analysis of PEC to podocyte regeneration in aging PEC-rtTA/LC1/R26R mice after UNx shows no significant changes of time (1.5 and 3 months, n=5; 6 months, n=4; 12 months, n=3). (E) Costainings of synaptopodin or claudin-1 (red) and the reporter eGFP-histone (green) on paraffin sections of control and experimental remnant kidneys 3 months after 5/6Nx using PEC-rtTA/H2B-EGFP reporter mice. No significant numbers of eGFP-labeled cells were detected within the glomerular tuft in any of the experimental or control groups. Arrowheads mark eGFP-positive cells along the vascular stalk. Original magnification, ×400. (F) Statistical analysis of eGFP-positive cells on the glomerular tuft 3 months after 5/6Nx (control, sham operated; pre-5/6Nx, resected kidney; 5/6Nx, contralateral remnant kidney after 3 months; n=6 in each group). (G) The absolute number of eGFP-positive cells on the tuft in glomeruli, with eGFP positivity on the tuft ranging only between 1 and 1.5. Error bars, SD. (H) Absolute number of eGFP-positive PECs on glomerular cross-sections, with eGFP positivity on the capsule showing that GFP-histone labeling persisted in PECs. Error bars, SD.