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. 2013 Nov 21;25(4):761–772. doi: 10.1681/ASN.2013060569

Figure 7.

Figure 7.

Effects of NMN and FK866 on NaPi-4 expression in OK cells. (A) Analysis of NAD concentrations in NMN-treated OK cells. OK cells are treated by NMN (0, 10, 100 µM) for 4 hours. (B) Crude membranes are immunoblotted using anti–NaPi-4. NMN is added to OK cells for 4 hours before collecting the crude membrane fraction. (C) Pi uptake assays are performed in the NMN-treated (100 µM) OK cells. The experiments are repeated three times. (D) Crude membranes are Western blotted and immunoprobed using anti–NaPi-4. OK cells are treated with FK866 (10 µM) for 12 hours before collecting the crude membrane fraction. (E) OK cells transfected with Nampt or empty vector. Crude membranes are immunoblotted using NaPi-4 antibody. FK866 (10 µM) or vehicle-treated OK cells for 12 hours before collecting crude membrane. NAM (10 mM) is added to OK cells for 4 hours before collecting crude membrane. Actin expression is used as an internal control. Data are presented as the mean±SEM (n=6/group). **P<0.01.