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. Author manuscript; available in PMC: 2014 Mar 28.

Published in final edited form as: Cell Growth Differ. 2000 Aug;11(8):417–424.

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Fig. 1 — RING3 kinase activity and protein level after serum stimulation of fibroblasts. Extracts were prepared at progressive times from 10⁷ serum-stimulated Swiss/3T3 cells and immunoprecipitated with αRING3 rabbit polyclonal antibody. Immune complexes were separated by SDS-PAGE, electroblotted to PVDF, and subjected to autophosphorylation assay (Ref. 1; IP kinase) or αRING3 immunoblot.