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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Stem Cells. 2014 Jan;32(1):135–144. doi: 10.1002/stem.1537

Figure 1. Expression analysis of 26S proteasome and immunoproteasome subunits by real-time PCR.

Figure 1

mRNA were extracted from sorted SUM159PT non-CSCs (ZsGreen-cODC) and CSCs (ZsGreen-cODC+). 1 µg of total RNA for each condition was treated with DNAse 1 and then reverse-transcribed into cDNA. 1µl of cDNA was used for each reaction of the semi-quantitative PCR. In CSCs mRNA expression levels for proteasome subunits were consistantly down-regulated. Normalized means ± SEM from 3 independent experiments are shown.

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