Figure 1. Expression analysis of 26S proteasome and immunoproteasome subunits by real-time PCR.

mRNA were extracted from sorted SUM159PT non-CSCs (ZsGreen-cODC⁻) and CSCs (ZsGreen-cODC⁺). 1 µg of total RNA for each condition was treated with DNAse 1 and then reverse-transcribed into cDNA. 1µl of cDNA was used for each reaction of the semi-quantitative PCR. In CSCs mRNA expression levels for proteasome subunits were consistantly down-regulated. Normalized means ± SEM from 3 independent experiments are shown.