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. 2014 Mar 21;5:100. doi: 10.3389/fphys.2014.00100

Figure 3.

Figure 3

Glucose uptake analysis of proliferating and differentiated C2C12 myoblast cultures treated with either three concentrations (5, 10, or 25 μM) of PUFA (AA, EPA, or DHA) or endocannabinoid (AEA, 2-AG, EPEA or DHEA) or with three concentrations (1, 2, or 5 μM) of cannabinoid receptor antagonist (AM630 or NESS0327) for 24 h. Values shown are means of 3 experiments performed in triplicate. In proliferating cells compared to the BSA vehicle control (gray shaded line), DHA, DHEA, EPEA, AM630, and NESS0327 had higher glucose uptake while AA, AEA, and 2-AG had lower glucose uptake. In differentiated cells compared to the BSA vehicle control, glucose uptake was lower for AEA and 2-AG treatments while all other treatments were higher. Data were analyzed by a One-Way ANOVA and Tukey's mean separation test at α = 0.05.The mean values and pooled s.e.m are shown in the supplemental materials Table S3.