FIG. 10.

Relative gene expression of collagen type I expressed by MSCs seeded on dPCL/ECM scaffolds. PCL/ECM scaffolds cultured in serum-free conditions (a) without growth factor supplements (−) and (b) with TGF-β3 (+). Numeric ratios represent the ratio of chondrocytes to MSCs used to generate the ECM coating the scaffold, while d7, d14, and d21 distinguish the three ECM maturities evaluated. Data were normalized to GAPDH expression and presented as fold change in collagen type I expression. Fold ratios are presented as mean±standard deviation (n=4). Symbols describing statistical significance are defined in Table 3 (p<0.05).