FIG. 4.

Nuclear fast red stained sections of dPCL/ECM scaffolds indicating the location of remaining cell nuclei within the devitalized scaffolds. The images displayed here represent scaffolds generated with the following chondrocyte to MSC ratios: (a–c) 1:0, (d–f) 1:1, (g–i) 1:3, (j–l) 1:5, and (m–o) 0:1. ECM was deposited for (a, d, g, j, m) 7, (b, e, h, k, n) 14, and (c, f, i, l, o) 21 days before freeze–thaw devitalization. 10× magnification. Scale bar in (a) indicates 100 μm and applies to all images.