FIG. 9.

Relative gene expression of collagen type II expressed by MSCs seeded on dPCL/ECM scaffolds. PCL/ECM scaffolds cultured in serum-free conditions (a) without growth factor supplements (−) and (b) with TGF-β3 (+). Culture duration was 21 days for all groups. Numeric ratios represent the ratio of chondrocytes to MSCs used to generate the ECM coating the scaffold, while d7, d14, and d21 distinguish the three ECM maturities evaluated. Data were normalized to GAPDH expression and presented as fold change in collagen type II expression. Fold ratios are presented as mean±standard deviation (n=4). Symbols describing statistical significance are defined in Table 3 (p<0.05).