IHCK are cocultured in the holistic mode for 5 days (A, B), 6 days (C, D), and for 9 days (E, F). IHCK are stained by IIF for cytokeratin 19 (CK19; A, C, E; green) and cytokeratin 12 (CK12; B, D, F; green), while cell nuclei are counterstained by DAPI (blue). Scale bars represent 50 μm. Color images available online at www.liebertpub.com/tec