FIG. 3.

UCs on different AM scaffolds observed with transmitted light inverted microscope. (A) The first day after seeding, single UCs attached on eAM (black arrows). (A) On the third day, the UCs on eAM already formed small islands. Gradually with the UC expansion, displacement of AECs was noted. Asterisks indicate the area of exfoliated AECs. (D, G) In the following days, the UC proliferation on eAM remained slow, but continuous. (B) On dAM, the first day after seeding, UCs formed large islands and (E) continued to proliferate until just confluence. (C) The fastest UC proliferation was noted on sAM, where already on the first day after seeding, UCs covered half of the scaffold surface. (F) After the first days, the UC proliferation on sAM decelerated and in the third week, the difference in UC growth between AM scaffolds was no longer significant. (G) Graph displays mean percentage of AM scaffold area covered with UCs and standard error, *p<0.05. Scale bars: 100 μm.