FIG. 5.

Histological, histochemical, and ultrastructural analyses of established tissue constructs. (A–C) Hematoxylin–eosin. The urothelium was established on all three different scaffolds. On eAM and dAM, the urothelium was two-to-four layered, on sAM three-to-five layered. (D–I) Immunolabeling with anti-collagen IV and anti-collagen VII and (J–L) TEM, showed that on the eAM and dAM scaffolds, UCs attached on to the AM basal lamina (white and black arrows on D, E, G, H, J, and K). On the sAM scaffold, the UCs formed the basal lamina (F), expressing collagen IV (white arrow on F), but not collagen VII (I). DNA (nuclei) is blue. Scale bars: (A–I) 20 μm, (J, L) 500 nm, (K) 200 nm. Color images available online at www.liebertpub.com/tec