Fig. 4. SRC-1 and GRIP1 potentiate transactivation through the HNF1 gene promoter.
Expression plasmids that encode SRC-1 (20 μg) or GRIP1 (5 μg) and a reporter construct that contains either the wild type HNF1 gene promoter (2.5 μg) or the promoter that lacks a functional HNF4-binding site (ΔA) (8) were cotransfected into HepG2 cells. Results are presented relative to wild type HNF1 promoter activity in the absence of cotransfection of either SRC-1 or GRIP1 (100%). These results represent the mean ± S.E. of at least three experiments.