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Culture substrate did not affect the POS phagocytosis or binding capacity of fhRPE but altered POS fragment size. Micrographs of rhodopsin-stained POS bound or internalized by fhRPE cultured for 4 weeks on (A) polyester transwells, (B) nonporous PCL, and (C) porous PCL. (D) Quantification of POS uptake and (E) fragment size using rhodopsin antibody-based fluorescence. Scale bar: 500 μm. **P < 0.01.
