Table 5. Comparison of Active Site Distances of ADHs Complexed with NAD and Fluoro Alcoholsa.
| structure, resolution, T | PDB entry | ligand | NAD C4N–ligand C | NAD C4N–203 CA | NAD C6N–203 CA |
|---|---|---|---|---|---|
| WT, 1.12 | 4DXH | TFE | 3.44 | 5.50 | 4.52 |
| V203A, 1.1 | 4NFS | TFE | 3.53 | 5.47 | 4.20 (pucker) |
| F93W, 2.0, 4 °C | 1AXE | TFE | 3.16 | 5.65 | 4.48 |
| V203A, 2.5 | 1AXG | TFE | 3.83 | 5.40 | 4.22 (tilt) |
| F93W/V203A, 2.0 | 1A71 | TFE | 3.54 | 5.44 | 4.32 (tilt) |
| WT, 1.14 | 4DWV | PFB | 3.37 | 5.41 | 4.51 |
| WT, 2.1, 4 °C | 1HLD | PFB | 3.36 | 5.40 | 4.53 |
| V207A, 1.2 | 4NFH | PFB | 3.29 | 5.46 | 4.47 |
| V203A, 1.1 | 4NG5 | PFB | 3.40 | 5.33 | 4.18 (pucker) |
a
The distances (Å) are averages of two subunits, in structures determined at 100 K unless noted otherwise.