Figure 6.

(A) SEM/SEI (magnification 6500×). Progression of osteocyte entrapment, which occurs centripetally as the basal floor of the endosteal surface grows upwards. The dorsal osteocyte membrane possessed crests and vesicles, suggesting development of a dorsal secretory territory during this phase of entrapment. (B) SEM/SEI OsO₃ + K₃[Fe(CN)₆] (magnification 2000×). Detail of two unfinished osteocyte lacunae after maceration with osmium tetraoxide and potassium ferrocyanide. The floor of the lacuna is almost flat, with the openings of the radial canalicula of the osteocyte undergoing entrapment. The lacunar edges appear undermined due to entrapment process centripetal progression. The holes on the bone surface correspond to openings of canalicula left by dendrites of the regular osteoblast layer (removed by maceration).