Figure 3.

Immunohistochemical detection of olfactory ensheathing cell markers, Vim (A-D) and p75NTR (E-H), in the olfactory system of zebrafish. (A) Lack of vimentin expression in the olfactory pathway at low magnification. Rectangular areas marked with OE, ON and OB are shown in (B), (C) and (D), respectively, at higher magnification. Chromatophores are indicated by curved arrows. (B) The olfactory mucosa (arrowheads) is unstained. A chromatophore (curved arrow) is visible. (C) The olfactory nerve displays immmunonegativity for vimentin. (D) In the olfactory bulb, the olfactory nerve layer (arrows) and glomerular layer lack immunoreactivity for vimentin. (E) Distribution of p75NTR immunostaining in the whole olfactory system. Rectangular areas marked with OE, ON and OB are shown in (F), (G) and (H), respectively, at higher magnification. Some chromatophores (curved arrows) are present in the epithelial lamina propria. (F) Slight p75NTR immunoreactivity (open arrowheads) in the lamina propria of the olfactory mucosa (arrowheads). Two chromatophores (curved arrows) are visible. (G) The olfactory nerve shows slight immunopositivity (open arrowheads). (H) In the olfactory bulb, p75NTR immunopositivity (open arrowheads) is slight to moderate in the olfactory nerve layer (arrows) and reduced in the glomerular layer. GL, glomerular layer; OB, olfactory bulb; OE, olfactory epithelium; ON, olfactory nerve. Scale bars: 100 μm.