Figure 4.

Immunohistochemical detection of the olfactory ensheathing cell marker, Gal-1 (A-D), in the olfactory system of zebrafish. (A) Distribution of Gal-1 immunostaining in the whole olfactory system at low magnification. Rectangular areas marked with OE, ON and OB are shown in (B), (C) and (D), respectively, at higher magnification. Some chromatophores (curved arrows) are present in the epithelial lamina propria. (B) The olfactory mucosa (arrowheads), which contains a chromatophore (curved arrow), is not immunostained. (C) In the olfactory nerve, the extracranial segment is unstained, whereas the intracranial segment shows strong immunoreaction (open arrowheads). (D) In the olfactory bulb, the olfactory nerve layer (arrows) and glomerular layer exhibit strong immunostaining (open arrowheads). GL, glomerular layer; OB, olfactory bulb; OE, olfactory epithelium; ON, olfactory nerve. Scale bars: 100 μm.