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. 2013 Oct 24;224(2):192–206. doi: 10.1111/joa.12129

Figure 5.

Figure 5

Negative controls for immunostaining specificity in the olfactory system of zebrafish, obtained by omission of the primary antibodies. (A) Omission of anti-GFAP antibody results in lack of staining in the olfactory nerve, olfactory nerve layer (arrows) and glomerular layer. (B) The epithelial lamina propria (arrowheads), which contains chromatophores (curved arrows), shows no immunoreaction as a result of anti-GFAP antibody omission. (C) Omission of anti-PSA-NCAM antibody causes immunostaining deficiency in the olfactory nerve, olfactory nerve layer (arrows) and glomerular layer. (D) Omission of anti-PSA-NCAM antibody results in immunonegativity of the olfactory mucosa (arrowheads). Some chromatophores (curved arrows) are displayed. (E) The olfactory nerve, olfactory nerve layer (arrows) and glomerular layer show lack of immunostaining due to anti-vimentin antibody omission. (F) In the olfactory mucosa (arrowheads), immunostaining deficiency results from anti-vimentin antibody omission. Chromatophores (curved arrows) are shown. (G). Omission of the anti-p75NTR antibody causes immunonegativity in the olfactory nerve, olfactory nerve layer (arrows) and glomerular layer. (H) Immunonegativity of the olfactory mucosa (arrowheads) ensues from omission of anti-p75NTR antibody. Curved arrows point out chromatophores. GL, glomerular layer; OB, olfactory bulb; OE, olfactory epithelium; ON, olfactory nerve. Scale bars: 100 μm.