Table 1.
List of primary antisera and secondary fluorescence conjugated antisera used for immunohistochemistry in the present study.
| Host animals | Dilution | Catalogue no. | Source | |
|---|---|---|---|---|
| Primary antisera | ||||
| ChAT | Goat | 1 : 250 | AB144p | Chemicon International, Temecula, CA, USA |
| GABA | Rabbit | 1 : 5000 | A-2052 | SIGMA BIO SCIENCES, MO, USA |
| GABAB-R | Guinea pig | 1 : 4000 | AB1531 | Chemicon International |
| NOS | Rabbit | 1 : 4000 | B220-1 | EURO-DIAGNOSTICA, Beijerinckweg, the Netherlands |
| NPY | Rabbit | 1 : 5000 | 6730-0204 | Biogenesis, England, UK |
| PNMT | Sheep | 1 : 5000 | AB146 | Chemicon |
| Secondary antisera | ||||
| Anti-goat IgG | Cy2 | 1 : 100 | 705-225-147 | Jackson ImmunoResearch, West Grove, PA, USA |
| Anti-guinea pig IgG | Cy3 | 1 : 250 | 706-165-148 | Jackson ImmunoResearch |
| Anti-rabbit IgG | Cy2 | 1 : 100 | 711-225-152 | Jackson ImmunoResearch |
| Anti-rabbit IgG | Cy3 | 1 : 250 | 711-165-152 | Jackson ImmunoResearch |
| Anti-sheep IgG | Cy3 | 1 : 250 | 713-165-147 | Jackson ImmunoResearch |
ChAT, choline acetyltransferase; Cy2, cyanine; Cy3, indocarbocyanine; GABA, gamma-aminobutyric acid; GABAB-R, GABAB-receptor; NOS, nitric oxide synthase; NPY, neuropeptide tyrosine; PNMT, phenylethanolamine N-methyltransferase.