Figure 6.

Treatments combining CBD and PAC produce additive to synergistic inhibition of breast cancer cell viability. Cell viability was measured using the MTT assay. (A) 4T1 and MDA-MB231-luc-D3H2LN (LN 231) cells were treated for 2 days with vehicle, CBD or PAC. Specific dose ratios of CBD and Pac where then combined in (B) 4T1 and (C) MDA-MB231-luc-D3H2LN cells. Cell viability (%) was calculated as the MTT product absorbance in the treated cells/control cells × 100. These data were used to calculate (D) CI values as described in Methods. A CI value of <1, 1 and >1 indicates synergism, additivity and antagonism respectively (Chou et al., 1993). Data are the mean of at least three independent experiments; bars, ±SEM.