Figure 7.

Concentration-dependent effects of TAS2R10 agonists on MCh- and 5HT-induced Ca²⁺ oscillations in ASM cells. Lung slices were treated with either (A) 400 nM MCh or (B) 1 μM 5HT followed by chloroquine (CQN; from 4 mice), quinine (QN; from 4 mice) or denotonium (DEN; from 2 mice) at the indicated concentrations. (C) The effects of chloroquine (from 3 mice) and quinine (from 3 mice) on Ca²⁺ oscillations stimulated by 0.4 μM (n = 13 and n = 16 respectively), 1 μM (n = 14 and n = 12 respectively) and 10 μM (n = 18 and n = 24 respectively from 3 mice) MCh. All data are expressed as the mean ± SEM Ca²⁺ oscillation frequency (spikes·min⁻¹). *P < 0.05, significantly different from the MCh or 5HT responses.