Figure 4.
Exendin-4 reduces macrophage infiltration into the liver. After 5 weeks of feeding a Western-type diet containing 0.4% cholesterol, mice were treated with exendin-4 (50 μg·kg−1·day−1) or vehicle (PBS) s.c. for 4 weeks. In the liver, mRNA expression of Mcp-1 (A) was determined as normalized to cyclophilin and hypoxanthine ribosyltransferase mRNA levels. Data were calculated as fold difference as compared with vehicle (A). Liver sections were immunostained for Mac-1, and Mac-1+ macrophages were quantified (B). Values are means ± SEM (n = 17 mice per group). *P < 0.05; **P < 0.01 compared with vehicle.