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. 2014 Jan 13;171(3):735–745. doi: 10.1111/bph.12491

Figure 3.

Figure 3

GILZ knockdown reduces glucocorticoid inhibition of hypoxia-induced COX-2 expression. (A) A549 cells were transfected with plasmid expressing shRNA targeting GILZ and pretreated with dexamethasone (0.1 μM) for 1 h before treatment with hypoxia for 24 h. Immunoblots were probed with a COX-2 antibody and reprobed with actin antibody (top). Total RNA from A549 cells were analysed for GILZ or COX-2 mRNA expression by qPCR (bottom). (B) A549 cells were transfected with COX-2-Luc reporter plasmid and/or plasmid shGILZ and treated as indicated. After treatment, luciferase expression was determined. Values represent the mean ± SD (n = 3). *P < 0.05. All experiments were repeated at least three times.