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. 2014 Mar 1;88(1):106–118. doi: 10.1016/j.bcp.2013.12.026

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 1 — Western blot analysis of Cyp3a2, Sec61α, Oatp1a1, Ntcp, Mrp2, and PDI in SER and RER isolated from Wistar and TR⁻ rat livers.

5 μg (Cyp3a2, Mrp2), 50 μg (Sec61α) or 100 μg (Oatp1a1, Ntcp) protein was loaded per lane and separated by SDS-PAGE as given in section 2.4. Samples were probed against Cyp3a2, Sec61α, Oatp1a1, Ntcp, Mrp2, and PDI as described in 2.4. PDI was used as loading control in SER and RER fractions. Basolateral membrane fraction (BLM) isolated from Sprague Dawley rat liver was used as positive control for Oatp1a1 and Ntcp expression. Canalicular (CM) membrane fraction isolated from Sprague Dawley rat liver was used as positive control for Mrp2 expression. The figure shows a representative Western blot of at least two independent SER and RER isolations.