Table 2.
E17βG uptake in absence and presence of ATP in Wistar SER and RER fractions.
| Incubation time |
SER |
RER |
||||
|---|---|---|---|---|---|---|
| -ATP |
+ ATP |
-ATP |
+ ATP |
|||
| pmol/mg protein | pmol/mg protein | % of -ATP | pmol/mg protein | pmol/mg protein | % of -ATP | |
| 15sec | 129 ± 7 | 116 ± 22 | 90 ± 17 | 136 ± 9 | 108 ± 9 | 80 ± 6 |
| 1 min | 138 ± 17 | 159 ± 18 | 115 ± 13 | 132 ± 5 | 135 ± 8 | 102 ± 6 |
| 5 min | 181 ± 20 | 188 ± 13 | 104 ± 7 | 173 ± 14 | 161 ± 14 | 93 ± 8 |
| 2 h | 270 ± 20 | 265 ± 8 | 98 ± 3 | 207 ± 11 | 223 ± 11 | 108 ± 5 |
Data of E17βG uptake in absence and presence of ATP at the given time points of incubation are shown for a representative sample of 2 independent experiments using vesicles from different vesicle preparations. Data are given as mean pmol/mg protein ± SD from measurements at least in triplicates.