Figure 8. .

DBD plasma–treated medium does not produce cyclobutane pyrimidine dimers or nucleic acid oxidation in explanted human corneas. Ex vivo human corneas were exposed to hydrogen peroxide (200 μM), UV light (20 J/m²), DBD plasma–treated KGM-2 (120 seconds), or mock treatment. The corneas were then incubated in fresh KGM-2 for 2 hours, flash-frozen in OCT compound, and processed for indirect immunofluorescence. (A) Cyclobutane pyrimidine dimers (CPDs) were detected with the TDM-2 antibody, and (B) oxidized nucleic acids were detected with the 8-OHdG antibody. Nuclei were counterstained with Höchst 33,258. Fluorescent images are overlaid with phase-contrast images in the merge. Bar: 100 μm. Representative data are shown. n = 2.