Table 1. Primers used in the present study.

Complementary sequences to ORFs are capitalized. Restriction sites are underlined.

Primer	Sequence
TcNMT-pTREX_s	5′-gaattcATGGCAGAAGAGGGTTCAGGTTTACATCAG-3′
TcNMT-pTREX_as	5′-ctcgagCTATAGCATGAACAATCCCACGTCACTTGG-3′
TcNMT-pET15b-TEV_s	5′-catATGGCAGAAGAGGGTTCAGGTTTACATCAG-3′
TcNMT-pET15b-TEV_as	5′-ggatccCTATAGCATGAACAATCCCACGTCACTTGG-3′
5′-UTR-NotI _s	5′-ataagaatgcggccgcGTGATCTTCTCAACAACAAAAATGGATGA-3′
5′-UTRHindIII/PmeI_as	5′-gtttaaacttacggaccgtcaagcttTCCTTCAAAAGGCGATCAAGTCCA- AAATTAC-3′
3′-UTR-PmeI/BamHI_s	5′-gacggtccgtaagtttaaacggatccGATGCGGGCGGAATTTAGGAGAGA- AGT-3′
3′-UTR-NotI_as	5′-ataagtaagcggccgcCCGCATCCAGCAGATGGATTAATCACCGT-3′