Fig. 1.

Transcriptional activation of glycolysis genes ENO2, PFKP, PFKFB4, and ALDOC. Left panels (black): Quantitative real-time PCR analysis was performed analyzing mRNA levels of ENO2, PFKP, PFKFB4, ALDOC, and TBP as reference gene and compared to microarray data (hatched bars). RNA was prepared from mature SGBS adipocytes after 3, 6 and 16 h of cultivation under hypoxic conditions (1% O2). Results represent three independent experiments each performed in triplicate and are expressed as mean values ± SD. Middle and right panels (grey): SGBS adipocytes were treated additionally with 30 and 90 μM of CAY10585 (HIF-1 inhibitor). It should be noted that the scale on the y-axis is logarithmic. Results represent two independent experiments each performed in triplicate. All data are expressed as mean values ± SD. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.