Figure 2.

Key Responses to cAMP Are Attenuated in pikI⁻ Cells

(A) Representative response of Lifeact-GFP-expressing cells to uniform stimulation with cAMP.

(B) Lifeact-GFP marking F-actin in pseudopods of randomly moving, chemotactically competent cells. See also Movie S2.

(C) cAMP production in response to stimulation with 2-deoxy-cAMP.

(D) Production of cGMP in response to cAMP stimulation of cells.

(E) ⁴⁵Ca²⁺ uptake by cells in response to cAMP stimulation.

(F) PI(3,4,5)P₃ levels before and after stimulation with cAMP.

(G) Quantification of the number of blebs produced per cell in response to cAMP stimulation.

All graphs show the mean of at least three experiments conducted on separate days; error bars represent ± SEM.