Figure 6.

Combined Effects of EB1 and XMAP215

(A) Superaveraged intensity profiles from 1 nM EB1-GFP in the absence (green data) and presence (orange data) of 150 nM XMAP215. The black lines are a global fit to both profiles with fixed kinetic rates (k₁ = 1.39, k₂ = 0.24, k₃ = 0) and different growth velocities (64 and 107 nm/s, respectively). The peak positions of the corresponding molecular distributions are shown in Figure 4D.

(B) Superaveraged intensity profiles from 20 nM XMAP215-GFP in the absence (blue data) and presence (orange data) of 150 nM EB1. Black lines are Gauss-lattice fits to the profiles.

(C) TIRF microscopy images of EB1-GFP (green) and XMAP215-GFP (blue) on Cy5-labeled microtubules (red) grown in the presence of GTPγS.

(D) Schematic illustrating the different maturation states and the effect of EB1 on their transitions.

Error bars are SE. See also Figure S6.