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. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: J Med Chem. 2012 Oct 26;55(22):10160–10176. doi: 10.1021/jm3012728

Figure 11.

Figure 11

(A) Comparison of the crystal structure of the β-tubulin–taxol complex (PDB 1JFF)59 with the homology model of SaFtsZ in complex with 13. The proteins, nucleotides, and compounds are depicted as described in Figure 6A. The orientation of protofilament growth is indicated. (B) Comparison of the impact of 13 and the antineoplastic drugs paclitaxel (taxol) and nocodazole on the polymerization of microtubule-associated protein (MAP)-rich porcine β-tubulin (70% β-tubulin, 30% MAPs) at 37 °C. The time-dependent A340 profiles of 2 mg/mL porcine β-tubulin are presented in the presence of vehicle (DMSO) only (black), 40 μg/mL 13 (red), 25 μg/mL taxol (blue), or 10 μg/mL nocodazole (green). Experimental conditions for the tubulin polymerization studies were 80 mM PIPES·NaOH (pH 7.0), 2 mM MgCl2, 1 mM EGTA, and 1 mM GTP.