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. 2014 Mar 17;24(6):630–637. doi: 10.1016/j.cub.2014.01.033

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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APC/C^Cdc20 Is Responsible for Anaphase Progression in Mouse Oocytes

(A) Time-lapse confocal microscopy images of oocytes expressing H2B-mCherry and securin-eGFP. Representative images from littermate control (Cdc20^f/f) and cdc20^Δ/Δ oocytes are aligned to show kinetics of securin-eGFP and chromosome movements. GVBD normalized time (in hr) is indicated. Scale bars represent 10 μm.

(B) Time-course measurements of securin-eGFP mean fluorescence intensity in control and cdc20^Δ/Δ oocytes (see also Figure S1).

(C) Oocytes harvested at the GV stage from Cdc20^f/f and Cdc20^f/fZP3-Cre mice were microinjected with securin-eGFP, H2B-mCherry, and the indicated mRNA in M2 medium supplemented with IBMX. Oocytes were then released in IBMX-free M16 medium supplemented with nocodazole (5 μM). Chromosome movements and kinetics of securin-eGFP were visualized by time-lapse confocal microscopy. Scale bars represent 10 μm.

See also Figure S1.