Fig. 1.

Protocol steps for manual passage of hiPSC using six well plates, T25 flasks and T175 flasks. The original six well plate protocol was scaled up for T25 and T175 flasks. Volumes of reagents and media were scaled up accordingly, the incubation time for T175 flasks increased and the 1 ml tip replaced by 10 ml pipettes. The key parameters were identified and are listed above.