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The four versions of the automated protocol showing the parameters changed in the protocols.
| Time for colonies to settle down by gravity after washing (min) | Height from bottom of flask to aspirate enzymatic solution (mm) | Speed of mix and final dispense of liquid in pipette to break colonies in final step (ml/s) | Split ratio | |
|---|---|---|---|---|
| Version A | 10 | 0.5 | 5 | 1 | 1:7 |
| Version B | 15 | 5 | 10 | 1 | 1:7 |
| Version C | 15 | 10 | 20 | 1 | 1:7 |
| Version D | 15 | 10 | 50 | 50 | 1:5 |
