Table 2.
Time for colonies to settle down by gravity after washing (min) | Height from bottom of flask to aspirate enzymatic solution (mm) | Speed of mix and final dispense of liquid in pipette to break colonies in final step (ml/s) | Split ratio | |
---|---|---|---|---|
Version A | 10 | 0.5 | 5 | 1 | 1:7 |
Version B | 15 | 5 | 10 | 1 | 1:7 |
Version C | 15 | 10 | 20 | 1 | 1:7 |
Version D | 15 | 10 | 50 | 50 | 1:5 |