Supplementary Fig. S2.

Fig. S2. In Falibene et al. (2012)’s study of 5-HT’s role in the consumption 621 of food by ants, 622 they found that 5-HT fed to ants was most effective at suppressing feeding 3.5 h after 623 feeding. To identify whether the time after injection affected the consumption, we tested 624 bees at 30 min and 3 h after head-injection and found that bees consumed significantly less 625 when they were tested 30 min after injection than if they had been tested 3 h later (Fig 1B, 626 2-way ANOVA, time main effect, F1,152 = 23.7, P < 0.001). At 30 min, the two doses were equally effective (lsd, P = 0.623); however, at 3 h, only the 10-2 627 M dose was effective at reducing feeding (lsd, 628 P < 0.010). N = 20/group.